There are two sets of servers, in separate VPCs. The dynatrace server is loaded in one server in one vpc and it was given public IP for making data from servers from other VPC to communicate.
In second VPC, i placed a collector to collect the data from all the servers within that VPC, and forward to Dynatrace server in first VPC.
I am able to configure (instrument) the web server (IIS); but the application server configuration is not working
Log file at collector, in second VPC:
2016-01-06 06:50:14  info [native] JVM started successfully
2016-01-06 06:50:19 [000000f0] info [native] New connection on port 50000 received
Did you follow the instructions to instrument an ASP.NET Application? You first need to install the Dynatrace Agent (which I assume you already have as the Web Server Agent works). For ASP.NET you also need to enable our .NET agent through our .NET Agent Configuration Tool. After that you need to restart IIS to make sure IIS gets all the configuration entries correctly.
Please have a look at my YouTube Tutorial on instrumenting ASP.NET Apps - that should help you: https://www.youtube.com/watch?v=57f4LMhBjVE&index=5&list=PLqt2rd0eew1bmDn54E2_M2uvbhm_WxY_6